human fetuin b Search Results


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Sino Biological human fetuin b
a. Periodic arrangement of the active site and prodomain are highlighted as yellow in the surface representation of an idealised meprin α helical segment. b. Example curve representing a series of first-order rates (determined by fitting the linear region of fluorescence versus time) at a given meprin concentration. The kcat/Km is determined from this graph for several meprin concentrations and in triplicate c. First order rate constants (k cat /K m ) of meprin α and variants for small fluorogenic peptide cleavage. Oligomeric state does not appear to affect rate of cleavage of small molecule substrate. d. Inhibitory constant (IC 50 ) of meprin α inhibitors. Determined by fitting and normalising the linear region of fluorescence versus time, in the presence of varying amount of inhibitor at a fixed meprin α concentration. e. Globular proteinaceous inhibitor, murine <t>fetuin-B,</t> and small molecule compound 10d were unaffected by oligomeric state. f. Proteolytic stability of meprin α and variants against trypsin and plasmin. Meprin α oligomers were more stable compared to lower-stoichiometric variants. g. Meprin α thermal stability measured by nanoDSF. Meprin α possess superior thermal stability compared to variants that lack either the disulphide bridge (most drastic) or helical interface interactions. All measurements are statistically significant to all others (p<0.0001, ****) unless otherwise indicated.
Human Fetuin B, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems anti human fetuin mouse monoclonal antibody
a. Periodic arrangement of the active site and prodomain are highlighted as yellow in the surface representation of an idealised meprin α helical segment. b. Example curve representing a series of first-order rates (determined by fitting the linear region of fluorescence versus time) at a given meprin concentration. The kcat/Km is determined from this graph for several meprin concentrations and in triplicate c. First order rate constants (k cat /K m ) of meprin α and variants for small fluorogenic peptide cleavage. Oligomeric state does not appear to affect rate of cleavage of small molecule substrate. d. Inhibitory constant (IC 50 ) of meprin α inhibitors. Determined by fitting and normalising the linear region of fluorescence versus time, in the presence of varying amount of inhibitor at a fixed meprin α concentration. e. Globular proteinaceous inhibitor, murine <t>fetuin-B,</t> and small molecule compound 10d were unaffected by oligomeric state. f. Proteolytic stability of meprin α and variants against trypsin and plasmin. Meprin α oligomers were more stable compared to lower-stoichiometric variants. g. Meprin α thermal stability measured by nanoDSF. Meprin α possess superior thermal stability compared to variants that lack either the disulphide bridge (most drastic) or helical interface interactions. All measurements are statistically significant to all others (p<0.0001, ****) unless otherwise indicated.
Anti Human Fetuin Mouse Monoclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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BioVendor Instruments fetuin a
a. Periodic arrangement of the active site and prodomain are highlighted as yellow in the surface representation of an idealised meprin α helical segment. b. Example curve representing a series of first-order rates (determined by fitting the linear region of fluorescence versus time) at a given meprin concentration. The kcat/Km is determined from this graph for several meprin concentrations and in triplicate c. First order rate constants (k cat /K m ) of meprin α and variants for small fluorogenic peptide cleavage. Oligomeric state does not appear to affect rate of cleavage of small molecule substrate. d. Inhibitory constant (IC 50 ) of meprin α inhibitors. Determined by fitting and normalising the linear region of fluorescence versus time, in the presence of varying amount of inhibitor at a fixed meprin α concentration. e. Globular proteinaceous inhibitor, murine <t>fetuin-B,</t> and small molecule compound 10d were unaffected by oligomeric state. f. Proteolytic stability of meprin α and variants against trypsin and plasmin. Meprin α oligomers were more stable compared to lower-stoichiometric variants. g. Meprin α thermal stability measured by nanoDSF. Meprin α possess superior thermal stability compared to variants that lack either the disulphide bridge (most drastic) or helical interface interactions. All measurements are statistically significant to all others (p<0.0001, ****) unless otherwise indicated.
Fetuin A, supplied by BioVendor Instruments, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cusabio human fetuin b fetub elisa kit
Serological Validation of COVID-19 Biomarkers (A) Overview of blood samples collection from cohort 3, including F (n = 40), S (n = 40), and M (n = 40) patients and H volunteers (n = 40). (B) The number of days between symptom onset and the sample collection with different time points. (C–G) Plasma levels of the indicated proteins from the samples of cohort 3 were detected via <t>ELISA.</t> Data points indicate the data of single patient that are presented as median with interquartile range (F, n = 40; S, n = 40; M, n = 40; H, n = 40). The center line within each box shows the median, and the top and bottom of each box represent the 75 th and 25 th percentile values, respectively. The upper and lower whiskers extend from the hinge to the largest and smallest value no further than 1.5 times the distance between the first and third quartiles, respectively. Data were analyzed by unpaired two-sided Welch’s t test. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. See also and .
Human Fetuin B Fetub Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems goat anti human fetuin a
Serological Validation of COVID-19 Biomarkers (A) Overview of blood samples collection from cohort 3, including F (n = 40), S (n = 40), and M (n = 40) patients and H volunteers (n = 40). (B) The number of days between symptom onset and the sample collection with different time points. (C–G) Plasma levels of the indicated proteins from the samples of cohort 3 were detected via <t>ELISA.</t> Data points indicate the data of single patient that are presented as median with interquartile range (F, n = 40; S, n = 40; M, n = 40; H, n = 40). The center line within each box shows the median, and the top and bottom of each box represent the 75 th and 25 th percentile values, respectively. The upper and lower whiskers extend from the hinge to the largest and smallest value no further than 1.5 times the distance between the first and third quartiles, respectively. Data were analyzed by unpaired two-sided Welch’s t test. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. See also and .
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R&D Systems human fetuin b duoset elisa development system
Serological Validation of COVID-19 Biomarkers (A) Overview of blood samples collection from cohort 3, including F (n = 40), S (n = 40), and M (n = 40) patients and H volunteers (n = 40). (B) The number of days between symptom onset and the sample collection with different time points. (C–G) Plasma levels of the indicated proteins from the samples of cohort 3 were detected via <t>ELISA.</t> Data points indicate the data of single patient that are presented as median with interquartile range (F, n = 40; S, n = 40; M, n = 40; H, n = 40). The center line within each box shows the median, and the top and bottom of each box represent the 75 th and 25 th percentile values, respectively. The upper and lower whiskers extend from the hinge to the largest and smallest value no further than 1.5 times the distance between the first and third quartiles, respectively. Data were analyzed by unpaired two-sided Welch’s t test. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. See also and .
Human Fetuin B Duoset Elisa Development System, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems elisa kit
Serological Validation of COVID-19 Biomarkers (A) Overview of blood samples collection from cohort 3, including F (n = 40), S (n = 40), and M (n = 40) patients and H volunteers (n = 40). (B) The number of days between symptom onset and the sample collection with different time points. (C–G) Plasma levels of the indicated proteins from the samples of cohort 3 were detected via <t>ELISA.</t> Data points indicate the data of single patient that are presented as median with interquartile range (F, n = 40; S, n = 40; M, n = 40; H, n = 40). The center line within each box shows the median, and the top and bottom of each box represent the 75 th and 25 th percentile values, respectively. The upper and lower whiskers extend from the hinge to the largest and smallest value no further than 1.5 times the distance between the first and third quartiles, respectively. Data were analyzed by unpaired two-sided Welch’s t test. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. See also and .
Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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OriGene sr308924
Serological Validation of COVID-19 Biomarkers (A) Overview of blood samples collection from cohort 3, including F (n = 40), S (n = 40), and M (n = 40) patients and H volunteers (n = 40). (B) The number of days between symptom onset and the sample collection with different time points. (C–G) Plasma levels of the indicated proteins from the samples of cohort 3 were detected via <t>ELISA.</t> Data points indicate the data of single patient that are presented as median with interquartile range (F, n = 40; S, n = 40; M, n = 40; H, n = 40). The center line within each box shows the median, and the top and bottom of each box represent the 75 th and 25 th percentile values, respectively. The upper and lower whiskers extend from the hinge to the largest and smallest value no further than 1.5 times the distance between the first and third quartiles, respectively. Data were analyzed by unpaired two-sided Welch’s t test. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. See also and .
Sr308924, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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OriGene retroviral untagged vector
Serological Validation of COVID-19 Biomarkers (A) Overview of blood samples collection from cohort 3, including F (n = 40), S (n = 40), and M (n = 40) patients and H volunteers (n = 40). (B) The number of days between symptom onset and the sample collection with different time points. (C–G) Plasma levels of the indicated proteins from the samples of cohort 3 were detected via <t>ELISA.</t> Data points indicate the data of single patient that are presented as median with interquartile range (F, n = 40; S, n = 40; M, n = 40; H, n = 40). The center line within each box shows the median, and the top and bottom of each box represent the 75 th and 25 th percentile values, respectively. The upper and lower whiskers extend from the hinge to the largest and smallest value no further than 1.5 times the distance between the first and third quartiles, respectively. Data were analyzed by unpaired two-sided Welch’s t test. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. See also and .
Retroviral Untagged Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Reddot Biotech human fetuin b (fetub) elisa kit
Serological Validation of COVID-19 Biomarkers (A) Overview of blood samples collection from cohort 3, including F (n = 40), S (n = 40), and M (n = 40) patients and H volunteers (n = 40). (B) The number of days between symptom onset and the sample collection with different time points. (C–G) Plasma levels of the indicated proteins from the samples of cohort 3 were detected via <t>ELISA.</t> Data points indicate the data of single patient that are presented as median with interquartile range (F, n = 40; S, n = 40; M, n = 40; H, n = 40). The center line within each box shows the median, and the top and bottom of each box represent the 75 th and 25 th percentile values, respectively. The upper and lower whiskers extend from the hinge to the largest and smallest value no further than 1.5 times the distance between the first and third quartiles, respectively. Data were analyzed by unpaired two-sided Welch’s t test. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. See also and .
Human Fetuin B (Fetub) Elisa Kit, supplied by Reddot Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GeneTex primary antibody against human fetuin-b gtx112260
Serological Validation of COVID-19 Biomarkers (A) Overview of blood samples collection from cohort 3, including F (n = 40), S (n = 40), and M (n = 40) patients and H volunteers (n = 40). (B) The number of days between symptom onset and the sample collection with different time points. (C–G) Plasma levels of the indicated proteins from the samples of cohort 3 were detected via <t>ELISA.</t> Data points indicate the data of single patient that are presented as median with interquartile range (F, n = 40; S, n = 40; M, n = 40; H, n = 40). The center line within each box shows the median, and the top and bottom of each box represent the 75 th and 25 th percentile values, respectively. The upper and lower whiskers extend from the hinge to the largest and smallest value no further than 1.5 times the distance between the first and third quartiles, respectively. Data were analyzed by unpaired two-sided Welch’s t test. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. See also and .
Primary Antibody Against Human Fetuin B Gtx112260, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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RayBiotech inc human fetuin b elisa kit
Correlation between <t>fetuin-B</t> levels in serum and follicular fluid. r = 0.703, P < 0.001
Human Fetuin B Elisa Kit, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a. Periodic arrangement of the active site and prodomain are highlighted as yellow in the surface representation of an idealised meprin α helical segment. b. Example curve representing a series of first-order rates (determined by fitting the linear region of fluorescence versus time) at a given meprin concentration. The kcat/Km is determined from this graph for several meprin concentrations and in triplicate c. First order rate constants (k cat /K m ) of meprin α and variants for small fluorogenic peptide cleavage. Oligomeric state does not appear to affect rate of cleavage of small molecule substrate. d. Inhibitory constant (IC 50 ) of meprin α inhibitors. Determined by fitting and normalising the linear region of fluorescence versus time, in the presence of varying amount of inhibitor at a fixed meprin α concentration. e. Globular proteinaceous inhibitor, murine fetuin-B, and small molecule compound 10d were unaffected by oligomeric state. f. Proteolytic stability of meprin α and variants against trypsin and plasmin. Meprin α oligomers were more stable compared to lower-stoichiometric variants. g. Meprin α thermal stability measured by nanoDSF. Meprin α possess superior thermal stability compared to variants that lack either the disulphide bridge (most drastic) or helical interface interactions. All measurements are statistically significant to all others (p<0.0001, ****) unless otherwise indicated.

Journal: bioRxiv

Article Title: Helical ultrastructure of the oncogenic metalloprotease meprin α in complex with a small molecule hydroxamate inhibitor

doi: 10.1101/2022.03.13.484121

Figure Lengend Snippet: a. Periodic arrangement of the active site and prodomain are highlighted as yellow in the surface representation of an idealised meprin α helical segment. b. Example curve representing a series of first-order rates (determined by fitting the linear region of fluorescence versus time) at a given meprin concentration. The kcat/Km is determined from this graph for several meprin concentrations and in triplicate c. First order rate constants (k cat /K m ) of meprin α and variants for small fluorogenic peptide cleavage. Oligomeric state does not appear to affect rate of cleavage of small molecule substrate. d. Inhibitory constant (IC 50 ) of meprin α inhibitors. Determined by fitting and normalising the linear region of fluorescence versus time, in the presence of varying amount of inhibitor at a fixed meprin α concentration. e. Globular proteinaceous inhibitor, murine fetuin-B, and small molecule compound 10d were unaffected by oligomeric state. f. Proteolytic stability of meprin α and variants against trypsin and plasmin. Meprin α oligomers were more stable compared to lower-stoichiometric variants. g. Meprin α thermal stability measured by nanoDSF. Meprin α possess superior thermal stability compared to variants that lack either the disulphide bridge (most drastic) or helical interface interactions. All measurements are statistically significant to all others (p<0.0001, ****) unless otherwise indicated.

Article Snippet: His-tagged recombinant human fetuin-B (11834-H08H) was purchased from SinoBiological.

Techniques: Fluorescence, Concentration Assay, Nano Differential Scanning Fluorimetry

a. Rigid body fit of murine fetuin-B/meprin β crystal structure (PDB 7AUW) to helical structure of meprin α. Arrangement of fetuin-B reveal monomers may pack into a slanted intercalated state that is not significantly prohibited by steric clashes. b. View of a tetramer of fetuin-B based on meprin α docking reveals potential interactions to form a higher-order inhibitory filamentous complex are possible. c. Side view of single fetuin-B dimer forms a “horseshoe” where putative interactions between inter-subunit fetuin-B domains may occur shown in (d), and (e). d, e. Models are not refined, rigid body fitting results in some minor clashes. f. The predicted oligomeric interface corresponds to an evolutionarily conserved interface revealed by ConSurf analysis. g. Side and top views of the cryo-EM reconstruction of human fetuin-B (red) in complex with meprin α (grey, black) at 3.7 Å resolution. Fetuin-B is observed to pack intimately within the meprin α active groove and intercalate as a secondary helix.

Journal: bioRxiv

Article Title: Helical ultrastructure of the oncogenic metalloprotease meprin α in complex with a small molecule hydroxamate inhibitor

doi: 10.1101/2022.03.13.484121

Figure Lengend Snippet: a. Rigid body fit of murine fetuin-B/meprin β crystal structure (PDB 7AUW) to helical structure of meprin α. Arrangement of fetuin-B reveal monomers may pack into a slanted intercalated state that is not significantly prohibited by steric clashes. b. View of a tetramer of fetuin-B based on meprin α docking reveals potential interactions to form a higher-order inhibitory filamentous complex are possible. c. Side view of single fetuin-B dimer forms a “horseshoe” where putative interactions between inter-subunit fetuin-B domains may occur shown in (d), and (e). d, e. Models are not refined, rigid body fitting results in some minor clashes. f. The predicted oligomeric interface corresponds to an evolutionarily conserved interface revealed by ConSurf analysis. g. Side and top views of the cryo-EM reconstruction of human fetuin-B (red) in complex with meprin α (grey, black) at 3.7 Å resolution. Fetuin-B is observed to pack intimately within the meprin α active groove and intercalate as a secondary helix.

Article Snippet: His-tagged recombinant human fetuin-B (11834-H08H) was purchased from SinoBiological.

Techniques: Cryo-EM Sample Prep

Serological Validation of COVID-19 Biomarkers (A) Overview of blood samples collection from cohort 3, including F (n = 40), S (n = 40), and M (n = 40) patients and H volunteers (n = 40). (B) The number of days between symptom onset and the sample collection with different time points. (C–G) Plasma levels of the indicated proteins from the samples of cohort 3 were detected via ELISA. Data points indicate the data of single patient that are presented as median with interquartile range (F, n = 40; S, n = 40; M, n = 40; H, n = 40). The center line within each box shows the median, and the top and bottom of each box represent the 75 th and 25 th percentile values, respectively. The upper and lower whiskers extend from the hinge to the largest and smallest value no further than 1.5 times the distance between the first and third quartiles, respectively. Data were analyzed by unpaired two-sided Welch’s t test. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. See also and .

Journal: Immunity

Article Title: Plasma Proteomics Identify Biomarkers and Pathogenesis of COVID-19

doi: 10.1016/j.immuni.2020.10.008

Figure Lengend Snippet: Serological Validation of COVID-19 Biomarkers (A) Overview of blood samples collection from cohort 3, including F (n = 40), S (n = 40), and M (n = 40) patients and H volunteers (n = 40). (B) The number of days between symptom onset and the sample collection with different time points. (C–G) Plasma levels of the indicated proteins from the samples of cohort 3 were detected via ELISA. Data points indicate the data of single patient that are presented as median with interquartile range (F, n = 40; S, n = 40; M, n = 40; H, n = 40). The center line within each box shows the median, and the top and bottom of each box represent the 75 th and 25 th percentile values, respectively. The upper and lower whiskers extend from the hinge to the largest and smallest value no further than 1.5 times the distance between the first and third quartiles, respectively. Data were analyzed by unpaired two-sided Welch’s t test. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. See also and .

Article Snippet: Human Fetuin-B (FETUB) ELISA Kit , CUSABIO , Cat#CSB-EL008598HU.

Techniques: Biomarker Discovery, Clinical Proteomics, Enzyme-linked Immunosorbent Assay

Journal: Immunity

Article Title: Plasma Proteomics Identify Biomarkers and Pathogenesis of COVID-19

doi: 10.1016/j.immuni.2020.10.008

Figure Lengend Snippet:

Article Snippet: Human Fetuin-B (FETUB) ELISA Kit , CUSABIO , Cat#CSB-EL008598HU.

Techniques: Infection, Enzyme-linked Immunosorbent Assay, Software

Correlation between fetuin-B levels in serum and follicular fluid. r = 0.703, P < 0.001

Journal: Journal of Assisted Reproduction and Genetics

Article Title: Serum and follicular fluid fetuin-B levels are correlated with fertilization rates in conventional IVF cycles

doi: 10.1007/s10815-019-01454-5

Figure Lengend Snippet: Correlation between fetuin-B levels in serum and follicular fluid. r = 0.703, P < 0.001

Article Snippet: Fetuin-B was assayed using a commercial sandwich enzyme-linked immunosorbent assay (ELISA) (Human Fetuin B ELISA Kit, RayBiotech, USA), following the manufacturer’s protocol.

Techniques:

Serum and follicular fluid fetuin-B levels in LF and HF groups. LF: low fertilization (2PN rate < 30%). HF: high fertilization (2PN rate > 70%).*P < 0.05

Journal: Journal of Assisted Reproduction and Genetics

Article Title: Serum and follicular fluid fetuin-B levels are correlated with fertilization rates in conventional IVF cycles

doi: 10.1007/s10815-019-01454-5

Figure Lengend Snippet: Serum and follicular fluid fetuin-B levels in LF and HF groups. LF: low fertilization (2PN rate < 30%). HF: high fertilization (2PN rate > 70%).*P < 0.05

Article Snippet: Fetuin-B was assayed using a commercial sandwich enzyme-linked immunosorbent assay (ELISA) (Human Fetuin B ELISA Kit, RayBiotech, USA), following the manufacturer’s protocol.

Techniques:

Serum fetuin- B levels in cycles with or without PPN zygotes. PPN: polypronuclear.*P < 0.05

Journal: Journal of Assisted Reproduction and Genetics

Article Title: Serum and follicular fluid fetuin-B levels are correlated with fertilization rates in conventional IVF cycles

doi: 10.1007/s10815-019-01454-5

Figure Lengend Snippet: Serum fetuin- B levels in cycles with or without PPN zygotes. PPN: polypronuclear.*P < 0.05

Article Snippet: Fetuin-B was assayed using a commercial sandwich enzyme-linked immunosorbent assay (ELISA) (Human Fetuin B ELISA Kit, RayBiotech, USA), following the manufacturer’s protocol.

Techniques:

The relationship between serum fetuin-B levels and immature oocyte rate in IVF cycles. IOR: Immature oocyte rate.*P < 0.05

Journal: Journal of Assisted Reproduction and Genetics

Article Title: Serum and follicular fluid fetuin-B levels are correlated with fertilization rates in conventional IVF cycles

doi: 10.1007/s10815-019-01454-5

Figure Lengend Snippet: The relationship between serum fetuin-B levels and immature oocyte rate in IVF cycles. IOR: Immature oocyte rate.*P < 0.05

Article Snippet: Fetuin-B was assayed using a commercial sandwich enzyme-linked immunosorbent assay (ELISA) (Human Fetuin B ELISA Kit, RayBiotech, USA), following the manufacturer’s protocol.

Techniques: